Vol. 7, Issue 6 (2019)
A technique to derive dikaryons through intra-specific compatible mating-pairs of Pleurotus florida
Author(s): Lokesh Taram, Ashok Kumar Koshariya, Indra Kumar, Sachin Dwivedi, Dr. CS Shukla, HK Singh and Dr. AS Kotasthene
Abstract: Mushrooms are macroscopic fruiting bodies of fleshy fungi, belonging to the class Basidiomycetes or Ascomycetes. Mushrooms are an important source of nutrition particularly in the cereal based diet of the vegetarian people. Oyster mushroom is considered as first in quality followed by button, shiitake, straw mushroom and black ear mushroom. From fresh fruiting bodies of Pleurotus florida 31 single basidiospore were derived and evaluate for mating type status of monokaryotic isolates by two point culture technique given by Kothasthane 2003. All monokaryotic isolates derived from one species were inoculated in the same slant (2-3cm. apart) in a culture tube and were observed for the formation of tuft. Total of 961 possible pairs of P. florida were made between the isolates. In the present investigation one pairs of isolate which formed a thick tuft at the region of confluence were identified as compatible are P flor 10 and P flor 28 the derivatives of Pleurotus florida and were tentatively given the mating type status as AxBx for P flor 10 and AyBy for P flor 28. Among this one pair we took total 15 replication for different quality parameters like number of flushes in one bag flush size and yield.
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How to cite this article:
Lokesh Taram, Ashok Kumar Koshariya, Indra Kumar, Sachin Dwivedi, Dr. CS Shukla, HK Singh, Dr. AS Kotasthene. A technique to derive dikaryons through intra-specific compatible mating-pairs of Pleurotus florida. Int J Chem Stud 2019;7(6):2479-2483.
