Vol. 7, Issue 6 (2019)
Genetic diversity among the landraces of kodo millet (Paspalum scrobiculatum l.) using issr markers
Author(s): Lalit Prasad Singh Rajput, Keerti Tantwai, Sajjan Kumar Pooniya and Koji Tsuji
Abstract: Kodo millet (Paspalum scrobiculatum L.) is having high nutritional value, dietary fiber, antioxidant activity as well as drought tolerance characteristics. Present study was conducted for assessing genetic diversity using ISSR (Inter Simple Sequence Repeat) markers among a total of 42 landraces of kodo millet collected from five districts of Madhya Pradesh (India) viz. Rewa, Betul, Chhindwara, Dindori, and Jabalpur. Ten ISSR markers amplified a total of 63 loci while 56 loci showed 88.88% polymorphism. Average number of bands per primer was found to be 6.3 whereas, average number of polymorphic bands per primer was 5.6. Maximum number of alleles (09) was scored by the primer UBC-886 followed by 08 by UBC-807 whereas; minimum number of alleles (04) was scored by the primers UBC-812 and UBC-816. Cluster analysis was done and a dendrogram was generated using UPGMA (Weighted Pair Group Method with Arithmetic Mean). The highest PIC (Polymorphism Information Content) value of 0.58 was observed by primer UBC-884 revealing 07 alleles among 42 accessions. Percentage of the number of polymorphic loci within population among the three regions, the highest frequency of polymorphism was found in the Dindori region (69.84 ± 22.22). The highest genetic diversity was observed in Dindori region three other in region.
Pages: 2529-2534 | 344 Views 140 Downloads
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How to cite this article:
Lalit Prasad Singh Rajput, Keerti Tantwai, Sajjan Kumar Pooniya, Koji Tsuji. Genetic diversity among the landraces of kodo millet (Paspalum scrobiculatum l.) using issr markers. Int J Chem Stud 2019;7(6):2529-2534.
