Vol. 8, Issue 1 (2020)
Effect of resistance inducers on biochemical characteristics of apple against Dematophora necatrix causing white root rot of apple
Author(s): Joginder Pal, Satish K Sharma, Monica Sharma and Sunita Devi
Abstract: White root rot caused by Dematophora necatrix Hartig is one of the most devastating soil borne diseases attacking apple plants worldwide. In the present investigation, a pot experiment was conducted during 2016 at Model research Farm of Dr. YS Parmar university of Horticulture and Forestry Solan (H.P.) to evaluate the effect of induced resistance (IR) chemicals viz., salicylic acid, benzothiadiazole-s-methyl ester, potassium oxalate, β-amino butyric acid, calcium carbonate, sodium salicylate and di-potassium hydrogen orthophosphate against white root rot pathogen. These inducers were assayed at 0, 7, 14 and 21 days of pathogen inoculation. The results revealed that the salicylic acid treated apple seedlings developed highest amount of total phenol (493.13μg/g) which was followed by benzothiadiazole-s-methyl ester (451.80μg/g) and β-aminobutyric acid (422.60μg/g) respectively. While the lowest amount of total phenol was recorded in calcium carbonate (296.27μg/g). However, the maximum increase in polyphenol activity in terms of change in absorbance min-1 g-1 fresh weight of leaf (0.601) was recorded in salicylic acid treated apple seedlings followed by benzothiadiazole-s-methyl ester (0.551) and β-aminobutyric acid (0.505). The study likewise in polyphenol oxidase also revealed the effectiveness of salicylic acid in enhancing the peroxidase activity being highest in salicylic acid (0.703) followed by benzothiadiazole-s-methyl ester (0.643) and β-aminobutyric acid (0.609) respectively. However, the least activity was registered in calcium carbonate (0.366). In similar trends, highest PAL activity in terms of change in absorbance min-1 g-1 fresh weight of leaf was registered in salicylic acid treated seedlings (0.942) followed by benzothiadiazole-s-methyl ester (0.880) and β-aminobutyric acid (0.820). While least PAL activity was observed in treatment with (0.450). The salicylic acid estimation was also conducted and was found higher in benzothiadiazole-s-methyl ester (57.07μg/g) followed by β-aminobutyric acid (51.87 μg/g) respectively. Interestingly, it was found that irrespective of sampling intervals, maximum content of above tested biochemical parameters was noticed at 7th days followed by 14th days of pathogen inoculation. Our findings suggest that enhancing resistance in apple seedlings before infection could be an innovative, safe and alternative control method against white root rot of apple.
DOI: 10.22271/chemi.2020.v8.i1k.8350
Pages: 730-737 | 634 Views 99 Downloads
download (7517KB)
How to cite this article:
Joginder Pal, Satish K Sharma, Monica Sharma, Sunita Devi. Effect of resistance inducers on biochemical characteristics of apple against Dematophora necatrix causing white root rot of apple. Int J Chem Stud 2020;8(1):730-737. DOI: 10.22271/chemi.2020.v8.i1k.8350
