Vol. 8, Issue 1 (2020)
Biochemical and molecular studies on effect of polymer seed coating of soybean
Author(s): GS Lodhi, RK Samaiya, Yogendra Singh and SK Dwivedi
Abstract: The present study was conducted at the Research Farm Adhartal, Department of Plant Breeding and Genetics, JNKVV, Jabalpur (M.P.) during Kharif season of 2016-17. Research experiment was laid out in a Randomized block design with five replications and four treatments i.e T0 No treatment or (Water + Thiram), T1- Polymer seed coating (Disco AG SP Red L-200) + Thiram + Carboxine, T2- Polymer seed coating (Disco AG SP Red L-200) + Thiram + Genius coat, T3- Polymer seed coating (Disco AG SP Red L-200) + Thiram + Mycorhiza comprised of soybean genotype JS JS 20-29. Five plants were randomly selected from each treatment and replications for Phenological and Physiological Parameters. Maximum protein % was registered in Treatments Polymer seed coating (Disco AG SP Red L-200) + Thiram + Genius coat (T2) (40.64), Treatments T3 (20.04) possessed the maximum fat % and showed differed significantly among them. The DNA amplification pattern revealed that a total number of 42 RAPD loci were amplified with an average of 7.0 loci per RAPD primer comprising 30 polymorphic loci (71.43%) and 12 monomorphic loci (28.57 %). The range of polymorphism was 50 % (OPA-01&OPA 02) to 89.0 % (OPA 07). Amplified product (band) size ranged from 300 bp (OPA-03) to 2800 bp (OPA-05). The no of bands ranged from 04 (OPA-02) to 09 (OPA -07).
DOI: 10.22271/chemi.2020.v8.i1ah.8605
Pages: 2238-2241 | 513 Views 95 Downloads
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How to cite this article:
GS Lodhi, RK Samaiya, Yogendra Singh, SK Dwivedi. Biochemical and molecular studies on effect of polymer seed coating of soybean. Int J Chem Stud 2020;8(1):2238-2241. DOI: 10.22271/chemi.2020.v8.i1ah.8605
