Vol. 7, Issue 6 (2019)
Radiation induced mutation for resistance/ tolerance against Fusarium oxysporum f. sp. ciceri in chickpea (Cicer arietinum L.)
Author(s): MG Patil, RV Mali, KT Apet and CV Ambadkar
Abstract: Chickpea (Cicer arietinum L.) is the third most important pulse crop grown throughout the world. Fusarium oxysporum f. sp. ciceri is a soil-borne as well as seed-borne fungus that is a serious threat, thereby inflicting heavy quantitative as well as qualitative losses in chickpea crop. In India, during 2017-18 Chickpea was cultivated on an area of 105.73 Lakh hectares with a production of 111.58 Lakh tonnes and productivity of 1056 kg/ha (Anonymous, 2018). Wilt of chickpea caused by Fusarium oxysporum f. sp. ciceri which one of the most prevalent and widely spreading disease in India. The pathogen is facultative saprophytic and it can survive as mycelium and chlamydospores in seed, soil and also on infected crops residues, buried in the soil for up to five to six years. The utilization of mutation breeding is a simple, cost effective and time saving method. Therefore, present investigation on “Radiation induced mutation for resistance against Fusarium oxysporum f. sp. ciceri (Cicer arietinum L.)” was aimed at identification of suitable mutant or a combination of mutants influencing resistance to Fusarium wilt in chickpea. The experimental material was consisted of the population of four selected cultivars of chickpea on the basis of popularity, high yield and disease reaction (JG 62, BDNG 798, JAKI 9218 and Vijay) grown in randomized block design at College of Agriculture Golegaon, during Rabi 2018-19. Dry seeds (10-12% moisture content) of JG 62, BDNG 798, JAKI 9218 and Vijay these varieties were irradiated with different doses of gamma rays (20 KR, 30 KR and 40 KR). Another set of presoaked seeds in distilled water (12 hrs.) were treated with ethyl methane sulphonate at different concentration (0.2%, 0.3% and 0.4%) prepared for 6 hrs. A portion of seeds irradiated at 20 KR and 30 KR gamma- ray doses were also treated with 0.2% and 0.3% EMS independently for 6 hrs. Present findings revealed that mutagenic treatments (20 KR, 30 KR, 0.2% EMS, 0.3% EMS, 20 KR+0.2% EMS, 30 KR+0.2% EMS) showed significant impact on various morphological and biological parameters of the plant habit. The effect of different doses of Gamma rays, EMS and combination of these treatments on the number of pods per plant, number of seeds per plant, seed yield per plant and 100 seed weight in M1 population of chickpea reveals that sufficient variability induced in genotypes for this character across the various treatments.
DOI: 10.22271/chemi.2019.v7.i6d.8664.1
Pages: 219-224 | 640 Views 97 Downloads
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How to cite this article:
MG Patil, RV Mali, KT Apet, CV Ambadkar. Radiation induced mutation for resistance/ tolerance against Fusarium oxysporum f. sp. ciceri in chickpea (Cicer arietinum L.). Int J Chem Stud 2019;7(6):219-224. DOI: 10.22271/chemi.2019.v7.i6d.8664.1
