Vol. 8, Issue 3 (2020)
Effect of partial deoxygenated extender on different post thaw incubation periods of physico-morphological and functional attributes during cryopreservation of buffalo spermatozoa
Author(s): B Balamurugan, SA Lone, R Selvarani, M Ramamoorthy, Abhishek Kumar, JK Prasad, MC Pathak, MR Verma and SK Ghosh
Abstract: The study was designed to explore the effect of partial deoxygenated extender of different post thaw incubation periods on physico-morphological attributes during cryopreservation of buffalo semen. Semen samples with mass motility of ≥4+ and individual progressive motility ≥ 80 collected from murrah buffalo bulls were utilized. Immediately after collection, a portion of semen was evaluated for progressive motility, hypo-osmotic swelling response (HOS response) and acrosomal intactness and other portion was divided into two equal groups. Group I was kept as control and diluted with Egg yolk-Tris-Glycerol (EYTG) with a maximum of 60×106 sperm/mL. Group II was diluted with partially deoxygenated EYTG extender. Both the groups were subjected to equilibration and freezing. Both the group of semen samples were evaluated at pre-freeze and post-thaw stage of progressive motility, viability, HOS response and acrosomal intactness. Post-thaw motility and viability was studied at 0, 15, 30, 45 and 90 minutes of incubation. At pre-freeze and post thaw stage, significantly (p<0.05) higher progressive motility, viability, HOS response and acrosomal intactness was noticed in group II as compared to group I. Post-thaw motility and viability was significantly (p<0.05) higher in group II than that of group I at 0, 15, 30, 45, 60 and 90 minutes of post-thaw incubation.
DOI: 10.22271/chemi.2020.v8.i3l.9325
Pages: 972-976 | 498 Views 85 Downloads
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How to cite this article:
B Balamurugan, SA Lone, R Selvarani, M Ramamoorthy, Abhishek Kumar, JK Prasad, MC Pathak, MR Verma, SK Ghosh. Effect of partial deoxygenated extender on different post thaw incubation periods of physico-morphological and functional attributes during cryopreservation of buffalo spermatozoa. Int J Chem Stud 2020;8(3):972-976. DOI: 10.22271/chemi.2020.v8.i3l.9325
