Vol. 8, Issue 3 (2020)
Effects of plant growth regulators on callogenesis, somatic embryogenesis and plantlet regeneration of Melia azedarach L.
Author(s): Belghali Loubna, Belkoura Ilham and Mazri Mouaad Amine
Abstract: We developed an efficient regeneration system through indirect somatic embryogenesis for Melia azedarach L. Different culture media with various plant growth regulator (PGR) combinations were evaluated. It was found that callus induction, proliferation and morphology depend strongly on the PGR combination of the culture medium. In fact, the callus induction rate varied from 7 to 100%. The highest callogenesis rate was observed on half-strength Murashige and Skoog (MS/2) medium supplemented with 3 mg/L 1-naphthalene acetic acid (NAA), 1 mg/L zeatin and 5 mg/L gibberellic acid (GA3). Calli were either white and friable, light brown and hydrated or brown and compact, with different proliferation potential. Somatic embryo formation was observed either directly on the explant or on the previously induced calli, depending on the PGR combination of the induction medium. The highest somatic embryogenesis rate was 29% in the calli induced on MS/2 medium supplemented with 3 mg/L NAA, 1 mg/L 6-benzylaminopurine (BAP) and 5 mg/L GA3. Callus induced on MS/2 medium containing 3 mg/L indole-3-butyric acid (IBA), 1 mg/L BAP and 5 mg/L GA3 showed a somatic embryogenesis rate of 5% while a similar rate was observed on the explants cultured on MS/2 medium containing 3 mg/L NAA, 1 mg/L zeatin and 5 mg/L GA3. However, in this latter case, somatic embryos were formed directly on the explant. After transferring somatic embryos to the germination medium, the germination rate was 51.28%. All germinated embryos were successfully converted into complete plantlets and showed normal growth and development.
DOI: 10.22271/chemi.2020.v8.i3s.9392
Pages: 1397-1401 | 415 Views 69 Downloads
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How to cite this article:
Belghali Loubna, Belkoura Ilham, Mazri Mouaad Amine. Effects of plant growth regulators on callogenesis, somatic embryogenesis and plantlet regeneration of Melia azedarach L.. Int J Chem Stud 2020;8(3):1397-1401. DOI: 10.22271/chemi.2020.v8.i3s.9392
