Vol. 8, Issue 3 (2020)
Acrosome integrity and mitochondrial membrane potential of frozen thawed buffalo semen treated with heparin binding protein
Author(s): Yanben M Kikon, K Loganathasamy, VS Gomathy, K Vijayarani and M Vengatachalam
Abstract: The experiment was conducted to study the sperm acrosome integrity and mitochondrial membrane potential (MMP) of frozen thawed buffalo semen treated with heparin binding protein (HBP). Buffalo semen straws from 10 bulls were procured from Central Frozen Semen Production and Training Institute, Hesseraghatta, Banglore-560088. The frozen straws were thawed at 37 ºC for 30 seconds and emptied into a 15mL sterile plastic centrifuge tube containing 1mL capacitation medium (control), addition of 25µg/mL (treatment I), 50µg/mL (treatment II) and 100µg/mL (treatment III) of HBP. The contents were incubated at 37 ºC for 2 hours. After incubation, sperm acrosomal integrity was assessed by Giemsa stain method. In control, HBP treatment I, II and III, 51.50% ± 1.29, 46.30% ±0.86, 43.40% ± 0.66and 42.15% ± 0.40 spermatozoa, respectively had intact acrosome. But, 48.50% ±1.29, 53.70% ± 0.86, 56.60% ± 0.66and 57.85% ± 0.40 spermatozoa in control, HBP treatment I, II and III respectively had lost acrosome. Significantly (P<0.05), higher percentages of spermatozoa lost their acrosome integrity in HBP treatments as compared to control. Sperm MMP was determined by JC-1 (5, 5’, 6, 6’ - tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide) stain technique. Significantly (P<0.05) higher percentages of spermatozoa in HBP treatment I (44.05% ± 0.61), II (45.35% ± 0.62) and III (45.05% ± 0.77) showed mitochondrial membrane potential in comparison with control (39.75% ±0.60).Insignificant difference in sperm MMP was observed among treatments. This study suggested that addition of HBP in capacitation medium induces sperm acrosome reaction and enhances mitochondrial membrane potential.
DOI: 10.22271/chemi.2020.v8.i3ai.9574
Pages: 2438-2443 | 367 Views 78 Downloads
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How to cite this article:
Yanben M Kikon, K Loganathasamy, VS Gomathy, K Vijayarani, M Vengatachalam. Acrosome integrity and mitochondrial membrane potential of frozen thawed buffalo semen treated with heparin binding protein. Int J Chem Stud 2020;8(3):2438-2443. DOI: 10.22271/chemi.2020.v8.i3ai.9574
