Vol. 7, Special Issue 6 (2019)
Amelioration of oxidative stress due to infection of Demodex canis in dogs with herbal extract of Moringa oleifera
Author(s): Sushil Kumar, Bipin Kumar, Nirbhay Kumar, SP Singh and Rajiv Kumar
Abstract: Moringa oleifera is a well-known plant herb, rich in valuable phytochemicals with medicinal as well as dietary importance. The present study was therefore to specifically investigate the in-vitro and in-vivo antioxidant properties of methanolic (80%) and aqueous extracts of M. olifera in canine demodicosis. 18 dogs positive for demodex mites in deep skin scraping were selected for the proposed study. All the dogs were randomly divided into 3 equal groups (n=6), adopting different therapeutic regimen. Group A treated with Doramectin @600mg/kg bw. sc. weekly alone; group Btreated with methanolic extract of M. oleifera along with same miticidal therapy and group C treated withN-acetyl-l cysteine @70 mg/kg BW orally daily as standard antioxidant along with same miticidal therapy.Moreover, six apparently healthy dogs as negative control group D.The DPPH (1, 1-diphenyl-2-picrylhyrazyl) free radical scavenging activity of methanolic extract of M. oleifera and aqueous extract of Moringa oleifera and N-acetyl-l cysteine were 78.86%, 65.53% and93.98%respectively. Twelve peaks were detected during a run time of 1 hour as depicted in the chromatogram (HPLC). Among all the peaks detected, six peaks representing six different compounds were eluted in substantial amount, at retention times of 1.891, 2.238, 2.894, 3.213, 40.683 and 50.200 minutes. The mean value of oxidative indices like LPOs, SOD, catalase, and GSH were 8.02±0.32, 2.08±0.06, 45.10±2.66 and 0.26±0.01 revealed significantly (p<0.05) high value LPOs &SOD, low level of catalase and GSH, shows increasing of oxidative stress in demodicosis. After treatment mean values of LPOs, catalase, SOD close to healthy control animals.
Pages: 388-391 | 517 Views 138 Downloads
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How to cite this article:
Sushil Kumar, Bipin Kumar, Nirbhay Kumar, SP Singh and Rajiv Kumar. Amelioration of oxidative stress due to infection of Demodex canis in dogs with herbal extract of Moringa oleifera. International Journal of Chemical Studies. 2019; 7(6): 388-391.
